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Hi, everyone,
I am working on refinement of 3.8A data. The structure was solved by
molecular replacement with phaser. The crystal belongs to spacegroup P43
with 2 copies of complex in ASU. Each complex contains four domains. It
seems that the MR solutions are correct, because it gave reasonable Z scores
(6.0, 10.8, 12.7, 11.3, 15.9, 14.3) for the six domains. It is very
difficult to find the last two small domains. So I just did rigid body
refinement and annealing, manually placed the remained two domains according
to the fo-fc map. Based on this solution, I performed the refinement with
refmac5, but the Rf and R (48%, 43%) are quite high.
Are there any suggestions on searching the lost domains and refinement on
such low resolution data. In this case, what is the acceptable values for Rf
and R?
Thank you very much!
zhihong
Institute of Molecular and Cell Biology
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