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hi all
i m refining structure at 3.2A.The problem that i m facing is the
difference between Rfree and Rcrys is decreasing. right now it is only
1.1%. i have tryed to improve the steriochemistry by looking at
Ramachandran but no success. whenever i increase the no. of
minimization steps, i always end up with an increase in Rfree.
plz tell me what other options one can try to improve difference
between Rfree and Rcrys.
Thanx in advance
vineet gaur

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