I would think a CC of 40 and and Rfactor of 45 was wonderful!
Eleanor
Vineet Gaur wrote:
Hi,
I'm trying to find a molecular replacement solution for a Fab
molecule. Have tried a few models with AMoRe. None of them seem to
have worked as yet. The best soln. till now has a correlation of 40
and a R factor of 45. I was told that this problem sometimes occurs
because of variable elbow angles in the Fab molecules and consequent
variable heavy and light chain domain interfaces. So I split my model
in to 4 fragments - Light and heavy chains and each chain into
variable and constant domains. Now used four models as input (to try n
simulate a hetro-tetramer) in AMoRe but the program gets stuck at a
stage with the following error message:
'fixed part coming from a 6 body traing output
orientations coming from a 6 body traing output
stop >>o2tndo << no solution selected
stop >> amore<< error in previous step'
would somebody please help me figure this out. Any suggestions to
solve the Fab molecular replacement problem in general??
Thanks
begin:vcard
fn:Eleanor Dodson
n:Dodson;Eleanor
email;internet:[EMAIL PROTECTED]
tel;work:+44 (0) 1904 328259
tel;fax:+44 (0) 1904 328266
tel;home:+44 (0) 1904 424449
version:2.1
end:vcard
