Sent: Tue 9/5/2006 12:22 PM
To: Brown,Raymond (BIDMC - Experimental Medicine)
Subject: Re: [ccp4bb]: spherulites
Hi Ray,
I was able to turn spherulites of a DNA-protein
complex into finicky plates
(5-10 x 200 x 200 um) that diffract (very
anisotropically) to 3.0A. and I've so
far been able to collect a
mostly-complete native set and solve it by MR (with
density for the DNA
visible). So while this may not be water into wine, think of
it as water into
grape juice.
Anyway, what I started with was additive seeding. This
helped me turn spherulite
fuzzballs into single itsy-needles. I then did
several rounds of additive
screening in an attempt to make the xtals larger
and more defined. I finished up
by pursuing many different oligo lengths
until I found one that yielded the
best-diffracting
plates.
Jacob
[EMAIL PROTECTED] wrote:
> Hi
all,
>
> I have now progressed from phase separation to
growing many nice
> spherulites, after many months of trying to
crystallize some RNA-protein
> complexes. Has anybody out there been able
to turn water into wine?
>
> If you have had any experience of
successfully changing spherulites into
> single crystals with PEG, MPD, HD
etc, then I would really like to hear
> about your success
story.
>
> Any practical suggestions or tips are
welcome?
>
> Cheers,
>
>
>
Ray
>
>
> [EMAIL PROTECTED] <mailto:[EMAIL PROTECTED]>
>
--
Jacob
Corn
The Berger Lab
UC Berkeley - Molecular and Cell
Biology
[EMAIL PROTECTED]
phone: 510-643-8893
fax:
510-643-9290
