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Post Doc Membrane protein biochemist 71-12048 AstraZeneca is one of the world's leading healthcare companies with approximately 60,000 employees. Our passion for creativity and innovation means that we are constantly working to improve the productivity and efficiency of our new drug delivery process - so we can bring better products to market faster and make an even bigger difference to patient's lives. The Structural Chemistry Laboratory at Mölndal, Sweden is a centre of excellence supporting the global AstraZeneca discovery organization. Its mission is to provide and exploit the use of information on protein structure throughout the drug design process. The laboratory has around 40 scientist working in the disciplines protein engineering, NMR spectroscopy and protein crystallography. AstraZeneca is now looking to build a network to focus on membrane proteins both for structural biology as well as for biopharmaceutical exploitation. The overall objective is to further increase knowledge about this important drug target class. Emphasis will be on membrane protein expression methods, protein purification and crystallization. 2 years Post-doc membrane protein expression and purification We are seeking a PhD in biochemistry to work on the expression and purification of a target set of membrane proteins in Pichia pastoris. This work includes preparation and screening of Pichia strains for the functional expression of membrane proteins. Subsequent, extraction and affinity purification of overproduced proteins. Functionality will be monitored using (radio-isotopic) ligand binding assays, hence experience with working in a isotope lab will be advanteous. Purified membrane proteins will be characterized using electron microscopy, NMR spectroscopically, crystallography and with other biophysical techniques, which may involve both internal and external collaborations. Candidates should have experience with membrane protein expression, purification and characterization. Familiarity with membrane protein crystallization will be advanteous as would be knowledge on GPCR's or ion channels. Extensive practical experience, analytical thinking, and effectiveness are essential characteristics. Candidates require excellent interpersonal skills and need to work efficiently on their own initiative as well as in a team. For more information, contact: Arjan Snijder, Phone +46-31-7663691, [EMAIL PROTECTED] Please send your application and CV marked "Post Doc Membrane protein biochemist 71-12048", via "Apply Now" no later than September 21, 2006. We will only handle applications received via our website.
