| Hi All, I am refining a protein-ligand structure using REFMAC. My ligand is a modified dinucleotide and is covalently bound to a sidechain of the protein. I have defined a library for the free ligand in REFMAC but I don't know how to enter the restraints for the covalent bond between the protein and the ligand in REFMAC. Is there a way to do this in CCP4i/REFMAC Thanks for your attention, Pascal F. Egea University of California San Francisco Department of Biochemistry and Biophysics Robert M. Stroud Laboratory Genentech Hall 600 16th street room S-414 CA 94107-2240 San Francisco www.msg.ucsf.edu |
