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You might want to add the command
SEPARATION CLOSE
and PROFILE OPTIMIZE
to your script.
The box size is calculated automatically depending on your resolution,
if you have data worse than 2.5 (yes, some people still collect that
sort of data :-) ), then you'll end up with 9 different box sizes
depending on the region of the detector larger ones at low resolution
and smaller ones at higher resolution. If you have data beyond 2.5 A,
then Mosflm uses 25 box sizes.
You can control how close to an overlap you want to get by the
Separation close command, but make sure that you actually don't measure
overlapped reflections. As a rule of thumb it's always wise to run
strategy with an overestimated mosaicity to avoid overlaps - but I guess
you already have the data and you are now stuck with it. Alternatively
you could use XDS if you really care for those spots.
Good luck,
Jürgen
sarvind mani wrote:
hello everyone
I have collected one data set for protein crystal, but spots at edges
are overlapped . can any one tell me how to reduce the box size in
Mosflm.i am using latest version of Mosflm.
Thanks in advance
regards
mani
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--
Jürgen Bosch
Howard Hughes Medical Institute and
University of Washington
Dept. of Biochemistry, K-426
1705 NE Pacific Street
Seattle, WA 98195
Box 357742
Phone: +1-206-616-4510
FAX: +1-206-685-7002
