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Hi Rebecca, We do the same, I prepare 2-3 drops of cryoprotectant on a microscope slide, draw the crystal through the oil and "wash" in successive cryo drops until I see no more oil coming off. Paraffin oil itself is mot a good cryo protectant, if you want to use paratone oil you can use the same procedure, or first get rid of the paraffin oil in artificial motherliquor drops, then tranfer to paratone. Hope this helps, Flip -----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Sudharsan Sridharan Sent: Tuesday, 12 September, 2006 16:39 To: Page, Rebecca Cc: [email protected] Subject: Re: [ccp4bb]: mounting crystals grown in microbatch plates *** For details on how to be removed from this list visit the *** *** CCP4 home page http://www.ccp4.ac.uk *** Dear Rebecca, I've mounted many crystals from microbatch and sitting drop plates and haven't found it diffucult to mount xtals from either ! (well, of course a bit difficult when i first started mounting xtals !!) typically I try to remove the oil by dragging the xtals gently in the cryoprotectant drop until i see the oil layer is removed (at much as i can judge visually !) it's a tricky job removing the oil completely depending on the cryo (eg. paratone !!!!) so great attention is needed in doing this part ! and I tranfer the xtals to a fesh drop of cryo before mounting. -Sid. -- Sudharsan Sridharan Dept. of Biochemistry Cambridge University 80 Tennis Court Road Cambridge CB2 1GA UK. Ph: +44-01223-766028
