Re: [ccp4bb]: Modified lysine?

[David Roper]

Dear All The Structures of two enzymes involved in bacterial peptidoglycan biosynthesis, MurD and MurE have carbamylated lysine residues present in their active sites. The MurE (pdb:1E8C) reference is Gordon E, Flouret B, Chantalat L, van Heijenoort J, Mengin-Lecreulx D, Dideberg O Crystal structure of UDP-N-acetylmuramoyl-L-alanyl-D-glutamate: meso-diaminopimelate ligase from Escherichia coli J. Biol. Chem. v276, p.10999-11006 Hope this is useful Yours David Roper >>> Richard Gillilan <[EMAIL PROTECTED]> 13/09/2006 20:23:06 >>> *** For details on how to be removed from this list visit the *** *** CCP4 home page http://www.ccp4.ac.uk *** A lysine sidechain in my structure seems to have a large elongated blob of density attached to the end in a T-shaped configuration. The buffer only contains ammonium sulfate and Lithium Sulfate. Is anyone aware of structures in which modified lysine has been observed? What kinds of modifications are possible? Richard Gillilan MacCHESS Cornell University