*** For details on how to be removed from this list visit the ***
*** CCP4 home page http://www.ccp4.ac.uk ***
Hi Ed,
I suggest the reading of the following truly valuable paper:
Tan S, Hunziker Y, Pellegrini L, Richmond TJ.
Crystallization of the yeast MATalpha2/MCM1/DNA ternary complex:
general methods and principles for protein/DNA cocrystallization.
J Mol Biol. 2000 Apr 7;297(4):947-59.
Roberto
On 15 Sep 2006, at 13:29, Ed Wright wrote:
*** For details on how to be removed from this list visit the ***
*** CCP4 home page http://www.ccp4.ac.uk ***
I am starting work on a protein that binds double-stranded DNA. In
other
dsDNA-protein stuctures I have noticed that some structures use
blunt-ended
DNA while others use sticky end DNA with 1,2 or 3 base G/C
overhangs. Is there
an advantage to using sticky-end vs. blunt-end DNA for
crystallization? Also,
what is the reason for using the G/C overhang?
---
Dr. Roberto Steiner
Randall Division of Cell and Molecular Biophysics
New Hunt's House
King's College London
Guy's Campus
London, SE1 1UL
Phone +44 (0)20-7848-8216
Fax +44 (0)20-7848-6435
e-mail [EMAIL PROTECTED]
