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Dear folks,
Again, a experimental question. I am working on protein DNA
complex. the protein which i am working binds to the DNA unspecifically
and i do have the native form of the strcture. Since, then i have been
trying to do DNA-protein complex.
Since, the protein binds unspecifically to DNA. The trial of
experiments goes exponentially higher, with that the cost of the
experiment also goes higher :-( .
There was lots of discussion earlier about the length of the DNA, sticky
ends, overhangs...etc.
But, my question comes from the area about purity.
How pure the DNA should be for the crystallization trials?
I used to buy DNA without much purification, This is as normal as
buying primers for cloning. So, the purity is not much high.
for eg. there are purification like
Hydrogel Purity Electrophoresis, High Performance Liquid Chromatography,
which i am not using.
So, how good the purity should be for intial trials?
might be in later case i could go for high purity DNA during the
optimization isn´t.
with regards
Rajesh......
