[ccp4bb]: aggregation

[Jenny]

Hi All,

I'm trying to purify a protein which is expressed in soluble form.It's a His-tag protein, so when I run His-column, it's really easy to clog.In the next step size exclusion purification, the protein come off at the void volume, which suggests that the protein is severely aggregated.Does any one have any suggestions that I can modify the  purification condition so as to avoid aggregation problem?Or can I add GuHCl during His column purification so that it won't clog so easily?Thanks a lot.

Jenny