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Hi all:
I am trying to crystallize a complex of two proteins by mixing equal
concentration of the two.
At equimolar ratio or at 10mg/mL, for example, I get a precipitate right
away after mixing.
These two form a tight complex and even in other experiments using gel
filtration we have seen precipiatation
and when run on a gel, we see two bands with appropriate molecular masses.
Molecular weight of one is around 56 kDa and the other one is 20kDa.
I would like to know of methods that one could try to keep the complex
in solution so that it can be crystallized.
Both are highly soluble proteins and can be crystallized individually
with out any difficulty.
Both form crystals using MPD at about 40%. Any help would be realy
appreciated.
Thanks a lot
subbu
