Dear Shivesh,
Is your solvent content or Mathew's coefficients are reasonable with your current model?
Did you check the packing? Is there any void among the molecules?
R-factor and R-free is seems to be reasonable with your resolution and also you mentioned that you have not seen any density for missing part. If you have missing domain in your model and you have not accounted that portion in the structure factor calculation that might be reflected in your R-factor and R-free.
So most likely that ordered domain might be crystallized and flexible domain might be cleaved off during crystallization, even though you used the full length protein.
Mass spectra on your crystal might give you more information.
cheers
vk
Kabaleeswaran Venkataraman
Arise, Awake and stop not until goal is Reached
- Swami Vivekanand
Is your solvent content or Mathew's coefficients are reasonable with your current model?
Did you check the packing? Is there any void among the molecules?
R-factor and R-free is seems to be reasonable with your resolution and also you mentioned that you have not seen any density for missing part. If you have missing domain in your model and you have not accounted that portion in the structure factor calculation that might be reflected in your R-factor and R-free.
So most likely that ordered domain might be crystallized and flexible domain might be cleaved off during crystallization, even though you used the full length protein.
Mass spectra on your crystal might give you more information.
cheers
vk
Arise, Awake and stop not until goal is Reached
- Swami Vivekanand
----- Original Message ----
From: shivesh kumar <[EMAIL PROTECTED]>
To: [email protected]
Sent: Tuesday, October 3, 2006 1:51:53 AM
Subject: [ccp4bb]: half of the structure missing
From: shivesh kumar <[EMAIL PROTECTED]>
To: [email protected]
Sent: Tuesday, October 3, 2006 1:51:53 AM
Subject: [ccp4bb]: half of the structure missing
Dear all,
At present we have 2.4A resolution.the protein is supposed to have
two domains separated by a 8-residues linker region with 3 glycines
which we have checked with DisEMBL.So,one domain is can be flexible.We
have done the mass spectroscopy of the protein solution itself and we
are trying to check with the crystals also.There is no tag in the
protein.We kept two acetate molecules and 13 water molecules and it has
two bound calciums.
ThanX in advance.
shivesh
