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Hi there, I have the following problem: I expressed a designed 67mer peptide/protein (7.8kd) which we believe forms coiled coils. On SDS-PAGE after crosslinking we see : monomer, dimer, trimer tetramer and pentamer. I'd like to get the trimeric form clean and so far I've used size exclusion chromatography superdex 75 column, it doesn't really work, the separation efficiency is practically non existent in this size range, also I suspect that the rod shape of the protein doesn't help here. Can anyone suggest a better separation method between the various multimeric forms? I'd like to get a homogeneous trimeric form. Thanks, Iris
