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Hi, fffear should work for this, assuming that your fragment is reasonably well-ordered in your crystal (it'll probably work on the experimental phases before solvent flattening if this is the case). However, it expects inputs in terms of amplitudes and phases, so you'll either have to use your reflection data or back-transform the map. Pete > Dear all, > > Short of the painful process of moving it by hand, what is the > currently most efficient way to place a large protein fragment (say > 350 amino acids) in an experimentally phased electron density map? > The resolution is 2.7Å and we can see some secondary structure > elements in the map. > > Thanks > Derek > -- > Derek Logan tel: +46 46 222 1443 > Associate professor fax: +46 46 222 4692 > Molecular Biophysics > Lund University > Box 124, Lund, Sweden > > > Pete Meyer Fu Lab BMCB grad student Cornell University
