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Dear All : Sorry for the non-CCP4 question. I am currently starting to work on Pichia expression for the production of human proteins for x-ray studies. I was wondering if any of you have experience in Pichia expression for structural studies, and would like to share some knowledge with me (I am a complete begginer in the field). I am interested in the expression of human nuclear proteins, and I would like to hear opinions and feelings about what system to use (intracellular vs secreted, constitutive vs inducible promoters, etc.). If any of you have some good references about the topic I would also appreciate. Many thanks for your help. Cheers Francisco ----------------------------------------- Francisco J. Enguita, Ph.D. Host-pathogen Interactions Group Macromolecular Crystallography Laboratory ITQB EAN, Av. da República 2781-901 Oeiras Portugal Phone : +351-21-4469663 Fax : +351-21-4433644 E-mail : [EMAIL PROTECTED] -----------------------------------------
