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Dear CCP4BB, Thanks for all of the suggestions re my Phaser problem. I should now turn off 'pessimistic mode' and call it "my Phaser solution". I mutated the top solution from Phaser to my sequence (very easy in COOT - thanks COOT people) and after a quick refinement at 2A I have a model with R/Rfree 45/49 and an omit map from SFCHECK looks pretty good. Now I need to go and build the extra bits - probably into a DM type map. Or do the "boring" Arp/Warp... Thanks to all. Ed -------------- ** Please note new contact details ** -------------- T.Edwards Ph.D. 8.111 Astbury Building Astbury Centre for Structural Molecular Biology University of Leeds, Leeds, LS2 9JT Telephone: 0 (+44) 113 343 3031 FAX: 0 (+44) 113 343 3167 Mobile: 0 (+44) 794 077 4012 Email [EMAIL PROTECTED] http://www.astbury.leeds.ac.uk/ > Dear BB, > > I have a high resolution native data set (1.7A) and a decent model > for MR (35% identity over 70 residues, 100 residues total in ASU). > Data scales well in P 3 2 1 (but not P6), and there are absences > indicating a screw axis along the 3-fold. > > I'm trying MR with Phaser using data from 15 - 3.0 A. > > Phaser does not produce solutions with Z scores over 5 for P 3 2 1 or > P 31 2 1. > However, in P 32 2 1 there is a solution with Z score of 8.57. A part > of the .sum file is attached below. The 2 solutions written out from > set 1 trial 3 both have high Rfactors in a rigid body refinement > (over 55%), so I'm assuming that is not a correct solution. > > I tried the MR protocol is CNS. The cross rotation function scores > look good, but not the translation function scores and the top > solutions all have Rfactors over 55%. > > There is no evidence of twinning. > > So... can anybody point me to something else I should look at/try? > Also - why is there no "third" solution for set 1 trial 3? > > Thanks > Ed
