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Hi,Sebastiano ,
In my opinion, the solutions with high clashes can not be the right
ones. You 'd better choice the other ones or change the model to serach
again.
GOOD LUCK!
LEO
Pasqualato wrote:
Hi all,
we're trying to solve a molecular replacement with phaser with data at
3.8 angstrom.
We only have half of the model (of a different species) to search for,
and have to look for four models in the asymmetric unit (not an
straightforward case, indeed).
The thing is, we start with not-so-nice Z scores for the first
molecule, then when the program tries to fit the second molecule, we
do obtain nice Z scores (even higher than 20) but with a huge number
of packing clashes (not less than nine - and indeed the models
superpose if we take a look at those solutions).
So my question is: are these Z scores indicating we're somehow near to
a good solution or do we have to consider as good only the (high) Z
scores of those solutions which show very few clashes?
Thanks a lot in advance,
Sebastiano
--
Sebastiano Pasqualato, PhD
IFOM
Istituto FIRC di Oncologia Molecolare
via Adamello, 16
20139 Milano
Italy
tel +39 02 574 303 325
fax +39 02 574 303 310
