Dear Dr. Swan,
I don't know what is generating them, but I have now received >30
copies of your E-mail. It would be really nice if you or CCP4 could
do something about this.
Best Wishes,
Laurence Pearl
On 1 Dec 2006, at 15:52, Michael K Swan wrote:
Hi All,
I have a question about processing in HKL2000.
I have a dataset which looks like it will go out to 2.5A but the
problem is that when I have integrated the 360 frames (180degrees)
to 2.4A I get a large number of rejects on scaling which exceeds
the 50000 limit. So is there any way to increase this limit or
another method of reducing the number of rejects?
I can get scaling to work if I either cut the resolution down to
3.0 or lower but alternatively if I exclude alot of frames and
leave say 90 then the rejects are below 50000 and the scaling
produces data that looks good to at least 2.6-2.5A according to the
I/sigma and an Rmerge <0.5 in the final shell, however the
completeness suffers due to the low number of frames.
I should also say that I am suspicious that one of the reasons for
all the rejects might be that in the centre of the pattern the
spots are of a good radial shape but towards the edge they become
more elongated. I tried using the elliptical spot feature of
HKL2000 but this doesn't seem to be ideal as the spots are not
always elongated in the same direction.
Any thoughts or suggestions would be much appreciated,
Mike Swan.
<michael.swan.vcf>
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Laurence H. Pearl
Section of Structural Biology, Institute of Cancer Research
Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
Phone +44-(0)20 7153 5422 : Secretary +44-(0)20 7153 5443
FAX +44-(0)20 7153 5457 : E-Mail [EMAIL PROTECTED]
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" Live Simply and do Serious Things .. " - Dorothy Crowfoot Hodgkin
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