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Dear All, I believe that there is another issue with the CCP4 bb in addition to multiple copies of e-mails. In my humble opinion, some of us (maybe all) do not seem to be receiving messages in their chronological order. My belief is based on the fact that I sometimes receive some responses before receiving the actual e-mail containing the related question for the CCP4 bb since a few days! Hence some strange, and sometimes frustrated exchanges today. I hope that I am not adding to the general confusion / boredom, but will help some puzzled CCP4 bb members take a step back and understand some "time-distorted" reactions of their fellow members. I would also be glad if we could investigate the extent of that particular issue as well (not a big problem I think most of the time, but definitely a true one when the conversation becomes "heated"). Sincerely, Ingrid Mechin sanofi-aventis mailstop N-103, 1041 route 202-206 Bridgewater, NJ 08807-0800 tel: + 1 908 231 3348 fax: + 1 908 231 3576 e-mail: [EMAIL PROTECTED] <mailto:[EMAIL PROTECTED]> -----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] Behalf Of James Stroud Sent: Monday, December 04, 2006 4:44 AM To: CCP4 bulletin board Subject: Re: [ccp4bb]: HKL2000 processing. *** For details on how to be removed from this list visit the *** *** CCP4 home page http://www.ccp4.ac.uk *** It seems it would be trivial to filter for this particular message, either at the recipient side or at the ccp4bb server side. I think this single message is becoming an annoyance for a lot of people. I'm very close to adding a specific filter to my mail program. James On Dec 1, 2006, at 3:11 PM, Nikolina Sekulic wrote: > *** For details on how to be removed from this list visit the *** > *** CCP4 home page http://www.ccp4.ac.uk *** > > > Hello, > > I've been receiving this very message multiple times yesterday and > today. Is there a problem with your server? > > Nikolina > On Dec 1, 2006, at 9:52 AM, Michael K Swan wrote: > >> Hi All, >> I have a question about processing in HKL2000. >> I have a dataset which looks like it will go out to 2.5A but the >> problem is that when I have integrated the 360 frames (180degrees) >> to 2.4A I get a large number of rejects on scaling which exceeds >> the 50000 limit. So is there any way to increase this limit or >> another method of reducing the number of rejects? >> I can get scaling to work if I either cut the resolution down to >> 3.0 or lower but alternatively if I exclude alot of frames and >> leave say 90 then the rejects are below 50000 and the scaling >> produces data that looks good to at least 2.6-2.5A according to >> the I/sigma and an Rmerge <0.5 in the final shell, however the >> completeness suffers due to the low number of frames. >> I should also say that I am suspicious that one of the reasons for >> all the rejects might be that in the centre of the pattern the >> spots are of a good radial shape but towards the edge they become >> more elongated. I tried using the elliptical spot feature of >> HKL2000 but this doesn't seem to be ideal as the spots are not >> always elongated in the same direction. >> Any thoughts or suggestions would be much appreciated, >> >> Mike Swan. >> <michael.swan.vcf>
