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Dear all, It seems I've timed my lastest problem well to recent discussions in the bulletin board. I have refined several structures with restrained refinement with TLS refinement in refmac. If I understand it correctly, the B-factors listed in the output PDB are residual Bfactors. That being said, I deposited the refmac outputted PDB directly (with Bs as listed) and now I wonder if that was the correct thing to do. What will happen, for example, if and when someone tries to download the PDB and structure factors to look at the maps using the electron density server or similiar? Also, the PDB staff told me that SFCheck is having problems with one of my PDBs (says it has high Rfactors, when the output Rs from refmac are in the high 20s). Could the SFCheck be having issues because of the residual Bs? I might we say significant drop in Rs using TLS refinement. How do you all deposit your TLS refined PDBs? Thanks Eric -- D. Eric Dollins C266 LSRC, Box 3813 Duke University Medical Center Durham, NC 27710 (919)681-1668
