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We had success in High5 insect cells, which may help you. Our methods and resources are listed in our paper: Carlson, CB et al. Structure of the calcium-rich signature domain of human thrombospondin-2. Nat. Struct. Mol. Biol. Oct 2005: 12(10):910-914. Good luck, C. Britt Carlson Department of Medicine University of Wisconsin, Madison [EMAIL PROTECTED] >>> Lari Lehtiƶ <[EMAIL PROTECTED]> 12/18/06 5:29 AM >>> *** For details on how to be removed from this list visit the *** *** CCP4 home page http://www.ccp4.ac.uk *** Posted on behalf of my collegue: ~Lari~ ________________________________________ Dear all, Does anyone have experience in producing Semet protein from drosophila S cells, if you had any problems (no proper labeling) would also be interested in hearing about it. thank you, Tommi [EMAIL PROTECTED] ________________________________________
