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Dear Vaheh,
I usually prepare the 2M solutions and then:
1. If a final basic pH is needed, I add malic acid to the imidazole
solution until the desired pH is achieved
2. If a final acidic pH is needed, I add the imidazole to the malic acid
solution until the desired pH is achieved
With best wishes
Pietro
--
Pietro Roversi
Sir William Dunn School of Pathology, Oxford University
South Parks Road, Oxford OX1 3ER, England UK
Tel. 0044-1865-275385
