Toluenes and derivatized benzenes may absorp into your plastic tray? Or into
the tape covering your tray? Just few other destinations.
It would make sense that if the binding of the 'drug' to the protein is tight,
then you do not need much in immediate contact, it will get there. The method
below sounds very promising to me.
Mark
-----Original Message-----
From: [EMAIL PROTECTED]
To: [email protected]
Sent: Mon, 22 Jan 2007 3:57 PM
Subject: Re: crystal friendly solvents that are useful for dissolving
hydrophobic small molecules?
So I've never actually tried this proposed extension to the idea, but:
I (and many others) have gotten small hydrophobics (toluene, iodobenzene etc.)
into proteins, and these things typically have very small partition
coefficients, and they aren't horribly volatile (that's why I am a little
partial to iodobenzene).
Why not saturate a small solution of your small molecule in iodobenzene and
just
add a few microliters on top; if the binding is tight enough you can pull it
through and not bug your protein with a denaturing co-solvent.
I've noticed that the iodobenzene does largely disappear overnight (in hanging
drop), I don't know if this is because of evaporation or the iodobenzene just
"falls" into the reservoir. Maybe stick to sitting drop.
-----Original Message-----
From: CCP4 bulletin board on behalf of Green, Todd
Sent: Mon 1/22/2007 12:40 PM
To: [email protected]
Subject: crystal friendly solvents that are useful for dissolving hydrophobic
small molecules?
Hello All,
I am trying to soak some crystals with a small molecule that is quite
hydrophobic. I am having trouble with solubilty of the small molecule. It will
dissolve up to about 1 mM in 100 % DMSO, but precipitates at concentrations of
less than 15 micromolar when the DMSO concentration is below 20 percent in my
crystal growth solutions(which are peg 4k, low pH, low salt). Can anyone
suggest
solvents other than DMSO which might help dissolve the inhibitor and might be
somewhat friendly to my crystals.
Thanks in advance-
Todd Green
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