Hi Shivesh,

Have you tried dropping your MPD concentration (ie. by increments of
~5%)?  Changing the pH may also result in larger crystals.

JP


On 1/30/07, shivesh kumar <[EMAIL PROTECTED]> wrote:
Dear all
Sorry for unrelated query.I have crystallized a protein which is at 40% of
MPD.But the problem is that I get several crystals but these are
small.Thecrystals diffract upto
3A.I welcome any suggestion to make the crystals big enough to diffract at
high resolution.What additives I can add???Thanx in advance.
Shivesh


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