No just common buffer substances: Tris, NaCl.
I think the Nitrogen I used for pressure filtration is solved. It took
a long time, the pressure was high (I think around 5 bar) and I was
stearing it.
Since then it was was in an NMR tube with an stamp on the fluid so that
I noticed every bubble.
Justin
Kris Tesh schrieb:
Justin,
What is in your solution? Could it be that you are using a salt that at
your pH equilibrates with the gaseous form? Ammonium Sulfate, Carbonate
salts and some buffers do this automatically.
Kris
-----Original Message-----
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Justin
Schmitz
Sent: Thursday, February 08, 2007 11:07 AM
To: [email protected]
Subject: Re: [ccp4bb] Good NMR Board
Thank you for all the replies.
@ Kris
It is not the problem of single gas bubbles, but of solved gas. I
prepared the sample in the middle of December and it still degases that
means bubbles appear. They can be removed but after a few days new ones
are coming up. I'm doing NMR and the shimming always gets worse if this
appears. So it is difficult to get a good 3D dataset if in the middle of
the measurement the sensitivity breaks down.
I'm searching for a way to simple degas the sample. I put it in normal
vacuum in tube, but that doesn't work good because of the small surface.
Enlarging the surface would create the risk of abstracting too much
water so that the concentration rises to fast and the Protein aggregates.
Is there another way?
Tomorrow I will try there syringe method.
Justin
