"David R. Cooper" <[EMAIL PROTECTED]> wrote: From: "David R. Cooper" <[EMAIL
PROTECTED]>
Subject: Re: [ccp4bb] AKTA prime
To: Frank Lee <[EMAIL PROTECTED]>
Date: Wed, 14 Feb 2007 15:13:18 -0500
Hello, Frank.
I seem to be having trouble posting directly to the bulletin board.
Perhaps you could forward this for me.
We have two AKTA Primes. They came with an external pressure regulator
attached that gave the system ~ 0.25 Mpa system pressure that Artem
mentioned (Hi Artem). It can be removed with no loss in performance and the
pressure will drop to ~ 0.05 for an empty system. We run XK 10/30 Superdex
columns (~30 ml) at .5 ml/min.
In my opinion, the largest drawback of the Prime is that it lacks a good
computer interface for programming. The default is that you have to push the
buttons on the front to program it. Our systems are in refrigerated
cabinets, so writing a more complex program means you have to have the door
open for a long time.
To circumvent this, I have written a web server that allows you to generate
programs for you. The forms generate a text file that can be saved in the
C:\UNICORN\Bin\Prime directory and then run using the "Run stored program /
from PC" option. My page has sections for writing generic programs
"break-point by break-point" or performing size exclusion or desalting
loops.
Anyone with a prime is welcome to use my "Akta Prime Program Generator" and
contact me if you need help.
See http://ginsberg.med.virginia.edu/akta.html
Overall, these are not ideal systems. If this is the only system that you
will have, consider a more robust system. If you are looking for something
to do affinity purrifications, size exclusion, desalting and even simple ion
exchange, it is a pretty good choice.
Regards,
David
-------------------------------------------
David Cooper, Ph.D.
University of Virginia
Molecular Physiology and Biological Physics
Jordan Hall, Box 800736
1300 Jefferson Park Ave
Charlottesville, VA 22908-0736
Phone: (434) 982-3151
Fax: (434) 982-1616
On Tue, 13 Feb 2007 14:16:33 -0800
Frank Lee wrote:
>
> Dear all,
>
> I need to decide between buying an AKTA prime and an AKTA FPLC from GE
>health care. I understand AKTA prime is a low-pressure system, but because
>it is too much cheaper than AKTA FPLC, it is still very attractive to me.
>
> I will mainly use it for Nickel columns and gel filtration columns, and I
>am worried about the latter. Is it true that using AKTA prime, you can only
>run the 24 ml Superdex 200 column at 0.1 or 0.2 ml/min?
>
> Could anyone who has used AKTA prime give me some feedbacks? I would
>appreciate it.
>
> Best,
>Frank
>
>
> ---------------------------------
> Need Mail bonding?
> Go to the Yahoo! Mail Q&A for great tips from Yahoo! Answers users.
.
-------------------------------------------
David Cooper, Ph.D.
University of Virginia
Molecular Physiology and Biological Physics
Jordan Hall, Box 800736
1300 Jefferson Park Ave
Charlottesville, VA 22908-0736
Phone: (434) 982-3151
Fax: (434) 982-1616
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