Hi, I dare say with an R-factor of 0.64, your MR solution is simply wrong. Just into the blue, you could 1) try various MR-programs (phaser, molrep, amore...) 2) try a different search model 3) try your best model but start with subdomains or chop off possibly flexible parts 4) have a look at the CaspR server http://www.igs.cnrs-mrs.fr/Caspr2/index.cgi 5) with crystals that give such good data try to get other pahse information (soaks, co-crystallisation, SAD (SAD at home), etc.) Good luck, Tim
-- Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A On Fri, 16 Feb 2007, Emmanuel Prata wrote:
Dear all, I have a data set of a protein soaked with sacarose, and the statistic data is good until the molecular replacement (Rmerge 0,07 (24%), I/Sigma 7 (2,7), completness (99,8 (98%)), and resolution 1,98A. After this step, I can not get the Rfactor (64%) to drop (I believe the spacegroup is correct). Could anyone give me suggestions? Thank you in advance, Emmanuel Prata
