You can also buy cDNA from various cell lines and tissues. A few
hundred dollars will get you enough for 50 or so PCR reactions. If
you have some idea where the genes are transcribed (kidney, liver,
whatnot) you can buy tissue specific cDNA. You can also buy pooled
cDNA.
We've successfully amplified quite a few genes from cDNA. It is much
cheaper than buying individual clones and you can keep it around for
the next, currently undecided, project. Clones are great, but only
good for one project.
We've used this approach for human, rat, mouse, chicken, and maybe a
few other projects.
Google searches work well for finding vendors, though I also like to
use Biocompare.
Good luck,
Cynthia
On Feb 20, 2007, at 4:29 AM, Miguel Ortiz Lombardia wrote:
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Hi Andrew,
Can you get cDNA from human cell lines? Perhaps one of your colleagues
can provide you with a few microliters. You can amplify from there all
the genes you need (provided they are transcribed in that cell line;
therefore, better use cDNA from two-three cell lines) We have followed
this approach quite successfully.
Cheers,
Miguel
Andrew Wong escribió:
Sorry a little offtopic...
We'r trying to clones a number of putative human proteins for
crystallization. Besides IMAGE clones from OpenBiosystem, is there
any
the cheaper way of obtaining human cDNA clones? OpenBiosystem is
okish,
~$100Cdn for each clone, but does get abit expensive when you start
getting alot.
- --
Miguel Ortiz Lombardía
Centro de Investigaciones Oncológicas
C/ Melchor Fernández Almagro, 3
28029 Madrid, Spain
Tel. +34 912 246 900
Fax. +34 912 246 976
email: [EMAIL PROTECTED]
www: http://www.ysbl.york.ac.uk/~mol/
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~
Le travail est ce que l'homme a trouvé de mieux
pour ne rien faire de sa vie. (Raoul
Vaneigem)
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____________________
Cynthia Kinsland, Ph.D.
Cornell University
Protein Facility Director
607-255-8844
