We have one protein (bacterial antitoxin HigA, 2ICT) that crystallizes in many conditions (30-40) at 0.3-0.6 mg/ml; the crystals diffract to 1.63 A. -- Mark Arbing, PhD
Email: [EMAIL PROTECTED] Tel: (212) 854-5236 Fax: (212) 865-8246 Dept. of Biological Sciences Columbia University 702 Fairchild Center, MC2437 1212 Amsterdam Ave., NY, NY, 10027 Quoting Tassos Papageorgiou <[EMAIL PROTECTED]>: > Just to add that in one case we were able to get suitable > crystals after using a > protein concentration of as low as 1.5 mg/ml (kapetaniou et al. > Acta Cryst. > 2005, F61, 479-481) and microdialysis to slowly remove the excess > of salt. > Lower concentrations (0.50-0.75 mg/ml) could also produce > crystals but they > were too small. > > Tassos Papageorgiou > > > > Quoting Patrick Shaw Stewart <[EMAIL PROTECTED]>: > > > Ronaldo > > > > I have a database of crystallization conditions extracted from > the > > PDB. I was able to parse the data to extract the concentration > of > > protein in around 900 cases. > > > > The lowest 9 protein concentrations were as follows: > > > > PDB ID TYPE DATE Protein conc, mg/ml > > 1EYM ISOMERASE. 07/05/2000 0.75 > > 1ADQ COMPLEX (IMMUNOGLOBULIN/AUTOANTIGEN) 18/02/1997 1 > > 1W5G FOUR HELIX BUNDLE. 06/08/2004 1 > > 1UU4 HYDROLASE. 15/12/2003 1 > > 1W2U HYDROLASE. 08/07/2004 1 > > 1DXP SERINE PROTEASE. 13/01/2000 1 > > 1DY8 SERINE PROTEASE. 18/01/2000 1 > > 1DY9 SERINE PROTEASE. 31/01/2000 1 > > 1DG6 APOPTOSIS. 23/11/1999 1.2 > > > > The maximum protein conc was 200 mg/ml (1BLF) > > > > My database goes up to October 2004. Janet Newman and Tom Peat > have > > done this job far more conscientiously and could probably give > you > > better and more up-to-date data. > > > > Patrick > > > > > > On 2/23/07, Ronaldo Alves Pinto Nagem <[EMAIL PROTECTED]> > wrote: > > > > > > > > > Dear all, > > > > > > As protein crystallization is the main topic of the day may I > include > > > another question? > > > > > > What was the minimun protein concentration reported with > success in > > > crystallization trials? > > > > > > I ask that because the protein I am trying to crystallize is > much less > > > soluble than the one mentioned in the lasts emails. > > > > > > Thanks > > > > > > Ronaldo. > > > > > > > > -- > A.C.(Tassos) Papageorgiou, PhD phone: +358 2 333 8012 > (office) > Senior Scientist, Group leader fax: +358 2 333 8000 > Turku Centre for Biotechnology E-mail: > [EMAIL PROTECTED] > BioCity, Turku URL: > http://www.btk.utu.fi/~apapageo > FIN-20521, Finland >
