Dear Lei,
just in case: would that be possible that you are encountering a
swapping, ie 3D domain swapping, behavior? To check this, have a look
at the electron density around the "crash". If it is continuous, you
might consider this possibility.
Regards.
Leo
On Mar 3, 2007, at 12:10 AM, thunderbird wrote:
Hi all.
I am trying to solve a structure with one molecules in the asu
through MR.
Using a not good data set at 3.0A and a structure with sequence
similarity of
37%, Phaser gave a result of z score 14.7 and LL-gain 220,
probably a right
solution.
But when I slightly modified the model I got from Phaser and used
it as an
input to run Phaser again, I got another solution overlapped partly
with the
first solution. I don't know how it could happen like that. And
the Rfree
factor could not go
below 0.43 in the following refinemet. I am wondering what is wrong
with it?
thanx in advance
Lei Yin
Lei Yin, Ph.D.
National Laboratory of Biomacromolecules
Institute of Biophysics
Chinese Academy of Sciences
No. 15 Datun Road, Beijing 100101, China
E-mail:[EMAIL PROTECTED]
Tel: 86-10-64888548
==========================
Chavas Leonard M.G., Ph.D.
Structural Research Center
KEK,PF. 1-1 Oho
Tuskuba, Ibaraki - Japan
---------------------------------------------
PHS: +81(0)29-864-5200 (ext: 2682)
e-mail: [EMAIL PROTECTED]
URL: http://pfweis.kek.jp/~leo
==========================
