Qing Xie wrote:
Hi,
I'm trying to get the difference map by subtracting the native
electron density map from the complex electron density map. MAPMASK
has a function of ADD/MULT, but I don't know how to use it?
Any other ways to attack this problem in real space?
Thanks in advance,
Qing
Yes - mapmask will do it providing you ADD SCALE -1 fpr one map then
MAP ADD
But there isa no adjustment for scaling one map to the other.
It is better to do a difference map use F1=Fcomplex and F2 = Fnative and
the phases/FOM from the model.
(see Map Utilities)
Make sure you have scaled F1 and F2 together (scaleit will do it) AFTER
calculating the PHIC/FOM.
Most SF generating programs (REFMAC, SFALL, maybe Clipper utilitiy?)
output a Fobs scaled to Fcalc so you need to rescale the other F again..
Eleanor
