Dear All,
We are working with a DNA binding protein with 4 Zn sites in in ASU. Using SnB
and then CNS we were able to get a map using MAD phasing and could visualize
the density for the double helix of the DNA but it was a left handed spiral
instead of the usual right handed one. The space group which yielded the result
was P3(2). We tried to flip the sites and change the space group to P3(1) but
we are not able to generate a sensible map. Any advice regarding the best way
to proceed will be very helpful. The resolution is around 3.2.
Thanks
Ruchi
