did you think about to do (or send your samples) for the edman degradation?
-----Original Message----- From: CCP4 bulletin board on behalf of Yong Tang Sent: Sat 3/31/2007 7:05 AM To: [email protected] Subject: [ccp4bb] (bigger) fragment identification of limited proteolysis w/ mass-spec Dear all, just a super dummy question: I treated a protein with trypsin, found the protein being degraded into two well-define fragments, ran a sizing column to find them co-elute, sent the peak fraction for mass-spec, got the two masses. Now here is the question - is there any program readily available for me to roughly identify these two (around 20K) fragments with the full-length sequence and these two masses, and of cause, with the fact that I use trypsin to cut it? I checked a lot of programs listed on Expasy to find them mostly dealing with smaller peptide length. Any information would be highly appreciated. Thanks and have a nice weekend, -yong
