I would also have to put in a vote for POPS if you know the protease specificity well. You can input everything you know about primary structure specificity (not secondary or tertiary) and search whole proteomes for cut sites or just your favorite protein (cut and paste sequence).

http://pops.csse.monash.edu.au/pops.html

On Fri, 6 Apr 2007, William Scott wrote:

Date: Fri, 6 Apr 2007 14:30:19 -0700
From: William Scott <[EMAIL PROTECTED]>
To: [email protected]
Subject: [ccp4bb] Summary: [ccp4bb] predicting protease cleavage sites in
    proteins?

Thanks, everyone!

This was a very popular answer:  http://us.expasy.org/tools/peptidecutter/

also

        http://www.cbs.dtu.dk/services/NetCorona/
        http://www.cbs.dtu.dk/services/NetPicoRNA/

and

If you are interested in the physiological, known cleavage sites, you
may also try one of the possible searches at the MEROPS database:

http://merops.sanger.ac.uk/cgi-bin/show_substrate


I asked this on behalf of my wife; hence the British sarcasm:

Begin forwarded message:

It was really helpful thank you. Ironic that you get answers from all over the world instantly when in two weeks I can't get a response from any of the geniuses here.


Again, thanks.

Bill

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