Yuan All other tRNA structures, free or engaged in complex with their cognate synthetase (as for Arnez & Steitz' structure), contain at least a pseudoUridine. Cytoplasmic tRNAs almost invariably contain the conserved sequence T(54)-PseudoU(55)-C(56) in the so-called TPsi-loop. There are many such structures in the PDB and the NDB.
Philippe Dumas IBMC-CNRS, UPR9002 15, rue Rene Descartes 67084 Strasbourg cedex tel: +33 (0)3 88 41 70 02 [EMAIL PROTECTED] -----Message d'origine----- De : CCP4 bulletin board [mailto:[EMAIL PROTECTED] la part de Yuan Lin Envoye : vendredi 27 avril 2007 03:46 A : [email protected] Objet : [ccp4bb] Structures with pseudo-uridine? Dear All, I solved a structure of a pseudouridylated RNA and am now starting to write it up. Except for the ribosome, I can only find one X-ray structure that contains pseudo-uridine (Arnez JG and Steitz TA, 1994, Biochemistry 33:7560-7567). I was wondering is there any X-ray structures that I am missing? Also, I am looking for the references on the effect of psi on RNA structure, and likewise have come up empty handed. Could anyone suggest some for me? Thanks very much! Best, Yuan ------------------------ Yuan Lin Graduate Student Biochemistry and Molecular Biology Johns Hopkins University Rm. W8702 BSPH 615 N. Wolfe St. Baltimore, MD 21205 Ph. 443-287-4988
