Hello, you could try to let phaser search for the first fragment, too. That way it should produce a file ending with '.sol' which you can pass to phaser for the second search (bottom at the ccp4-gui, "Define search sets...").
Otherwise, if you do not want to move the first fragment, both the Euler angles and the translation should be 0 0 0. Tim On Friday 18 May 2007 11:24, Jay Thompson wrote: > Hi, > > I have a question with molecular replacement using Phaser. I'm trying to > solve a complex and I have a partial molecular replacement solution solved > using another program. This solution is correct and makes up ~50% of the > entire complex. I wanted to fix this solution and search for another small > fragment of the complex using Phaser. I've been reading the Phaser manual > and it seems that I cannot input a pdb with this partial solution and tell > the program to fix this molecule. It seems that fixed solutions can be > only input by putting in its Euler angles and fractional coordinate > translations. Is this correct that I cannot input a pdb and fix it? If I > cannot do this, then is there a quick way to identify the euler angles and > coordinate translations for Phaser. > > Thanks a lot!! > > Jay -- Tim Grune Australian Synchrotron 800 Blackburn Road Clayton, VIC 3168 Australia
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