Senility is, in fact, setting in. It was a modified version of CAD! Sorry for the confusion.
To further compound the confusion, I am not sure if my solution is what you are really looking for, although I suspect you should try it. What we did was use an overall negative B-factor to achieve a sharpening effect (Cell 119(3):393-405). Others had done this before (David Borhani comes to mind)....anyway, it can be very, very useful. Steve -----Original Message----- From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Soisson, Stephen Michael Sent: Wednesday, June 13, 2007 1:23 PM To: [email protected] Subject: Re: [ccp4bb] B-factor sharpening in CCP4 or Coot Eleanor was kind enough to modify truncate years ago for me to do this - I would guess the feature is still there. Thanks again Eleanor! Steve -----Original Message----- From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Jeff Lee Sent: Wednesday, June 13, 2007 1:19 PM To: [email protected] Subject: [ccp4bb] B-factor sharpening in CCP4 or Coot Hi, I have a question for everyone. I wanted to up-weight my high resolution terms in my electron density map by applying a B-factor sharpening term. I have mtz files produced from DM and I usually use Coot to generate my electron density maps. I was wondering is there an easy way to apply a B-factor sharpening term in Coot? If not, is there a program in ccp4 where by I can set the B-sharp factor? Thanks Jeff ------------------------------------------------------------------------ ------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------ ------ ------------------------------------------------------------------------ ------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------ ------ ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------------
