2 methionines in 63 amino acids might work. However, if I'm reading your message correctly, the methionines are likely to be located in disordered loops, which makes them less of a good bet.
One alternative to consider is using modified DNA oligos, e.g. with 5-iodo-deoxyuracil or 5-bromo-deoxyuracil substituted for thymine or with 5-iodo or bromo cytosine. These are pretty widely available (e.g. from www.oligos.com), and not particularly expensive. You can use them to solve by SAD/SIRAS or MAD in the case of Br derivitives. Quoting "W.M. B." <[EMAIL PROTECTED]>:
Hi All I have a protein-DNA complex dataset, and can't solve it by MR. I would like to hear your advise whether I should prepare the selenomet protein. The native protein is homeodomain protein, which has 63 AA. It only has two Met in the cloning artifacts. There are two complexes in the AU. Will MAD be working? Did anyone have similat experience? Thanks for your response. Best Steve
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