Hi, give Ta6Br12 a try - it is a cluster with an incredible white line - really nice for phasing :-). But the best thing is that it does not mind high ionic strength (worked great for me). There are a couple of references out for it which you can check for more details...
Good luck, Petra > Hello all, > I am struggling with getting the phases out for my protein. Heavyatom > database shows that only mercury and samarium have the binding motifs in > it. > > Sodium iodide soak is killing the crystal even as less as 0.2 Molar > concentration for 30sec. > With Potassium iodide crystal is okay upto 0.75M for 1 & a half min but > the > incorporation is not at relevent sites.(Iodide signal is not distinct and > is weak) > Hg & Au salts precipitate it. > With Pt signal is weak. > Somewhere I read that high salt causes problems for heavy atom to bind to > protein.My protein needs high salt for stability (750mM NaCl) > > Please help with some suggestion!! > -- Petra Verdino, PhD Research Associate The Scripps Research Institute, BCC206 10550 North Torrey Pines Road CA 92037 La Jolla, USA tel:1-858-784-2294 fax:1-858-784-2980
