>If you want to combine though, I use the molrep phases to get all >heavy atoms >in an anomalous fourier map, then go to SHARP with these sites and >use the 'External phases' option >in the SHARP gui. Works well for me.
This has worked well for me too...I highly recommend SHARP. Good luck- Steve -----Original Message----- From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Anastassis Perrakis Sent: Tuesday, July 17, 2007 12:56 PM To: [email protected] Subject: Re: [ccp4bb] Combining MR and MAD phases I would be tempted to say that you don't need to combine; with 1.7 A data, refinement should do it with no trouble. And, yes, of course ARP/wARP (7.0) would be the best way to do it ;-) If you want to combine though, I use the molrep phases to get all heavy atoms in an anomalous fourier map, then go to SHARP with these sites and use the 'External phases' option in the SHARP gui. Works well for me. I also recall that Phaser has an option for exactly do that, but with SAD data only. But, again, if you have 1.7 A data good old refinement and auto- building will (likely) do the job in a glance. Tassos PS If you dont want to install arp/warp locally why not try: http://cluster.embl-hamburg.de/ARPwARP/remote-http.html PS2 Apologies for the shameless promotion of ARP/wARP ;-) On 17 Jul 2007, at 18:22, Joe Batchelor wrote: > Hi, > > I have a 1.7 A native dataset, a good MR solution for 2/3 of the > protein, and MAD phases to 3 A. How should I combine the MR phases > with the MAD phases? > > Thanks, > Joe ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------------
