It might be worth it to try phaser

Wu, Mousheng wrote:
> hi, everyone!
>
> I am struggling with my crystal structure in a very big unit cell. my
> protein is about 16KDa. probably there are about 20 molecules in the
> asymmetric unit. I tried to use molecular replacement to solve the
> structure. However, there is only one NMR structure available for me. The
> second trouble is there are so many molecules in the ASU.  I tried use NMR
> structure as a model to solve it. MolRep gave me 4 solutions. I refined it
> first and see the map. The map looks quite reasonable. some parts of the
> model fit the density and some do not . i tried to trace one molecule
> first. you can image how hard it is.
>
> Does anyone have such experience?  how can i find so many solutions with
> NMR model? your suggestions are much appreciated!
>
> Mousheng Wu
>

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