Every modern structure should be "above average" by procheck criteria.
For modest resolution structures, the availability of ncs restraints
should have a big effect on one's Ramachandran expectations: I've
found ncs restraints at 3-3.5A do wonders for the Ramachandran plot
even when they don't do much for Rfree.
Phoebe
At 11:44 AM 8/3/2007, Edward Berry wrote:
Procheck puts out such a correlation (% most favorable
vs resolution) in the _04.ps file. For example look at
page 7, first panel of the sample procheck output at:
http://sb20.lbl.gov/SQR/procheck-2H88.pdf
It appears that 83.5% would be well above average
for a 3 A structure according to procheck statistics.
(However I think most structures nowadays are
"above average" by procheck statistics)
Ed
Xiaofei Jia wrote:
Dear all,
I am now preparing my structure for deposition. The
crystal diffracts to 3.0 A. R: 0.20; R free: 0.25.
What I am concerned with is the Ramachandran plot;
83.5% in core region, 15.8% in allowed, 0.5 % in
general allowed,0.2% in disallowed region. The model
fits in density pretty well and all the attempts to
improve Ramachandran have not been successful after
quite a few trials. I am wondering if 83.5 % in core
region is acceptable with 3.0 A resolution data? Moreover, is there
some numeric correlation between
diffraction resolution and model Ramachandran? Thank
you for your help.
Xiaofei
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Phoebe A. Rice
Assoc. Prof., Dept. of Biochemistry & Molecular Biology
The University of Chicago
phone 773 834 1723
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