It seems likely that you could even monitor the heme group somehow, depending
on its
characteristics. I would bet that when the protein denatures, the
released/exposed heme's
spectroscopic properties change in some way. I would just see what happens with
the heme itself...
Jacob
==============Original message text===============
On Tue, 11 Sep 2007 6:42:57 pm CDT Jan Schoepe wrote:
Dear all,
To optimize my crystallization conditions I would like to do thermal melts but
unfortunately, there
is heme in the molecule which messed up my experiments. The dye I used is
colored orange ("SYPRO
orange"). Has anybody an idea if it is possible to get serious results with an
appropriate dye
instead or does Thermal melt never work with heme in the protein? Many thanks!
Jan
---------------------------------
Die etwas anderen Infos rund um das Thema Reisen. BE A BETTER
WELTENBUMMLER!===========End of
original message text===========
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Jacob Keller
Northwestern University
6541 N. Francisco #3
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(847)491-2438
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