Hello everyone,
I am asking for some help with my soaking experiment, my protein should bind
with oligosaccharide such as maltotriose,tetraose,...
the crystalization condition is 0.1 M HEPES, ph 7.2. I am having trouble
finding stabilization buffer: it does not like PEG, ( it dissolved in PEG
solution faster than in pure water) , I also tried glycerol, which is not
better. I finally use 10% MPD, but when I add ligand ( oligosaccharide), from
several mM to 50 mM , the crystals can only last from 12 hrs to 24 hrs before
crack. this make the soaking really hard.
by the way,it seems that the crack is associated with adding ligand, ( without
ligand,it could last several days), is that because the binding destroyed the
crystal?
anyone have suggestion for this situation ? Thanks a lot
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