Yes to keeping the same FreeR
Bfactors - I doubt if it matters, but I usually start a new data set by
setting Bs to the Wilson suggestion..
Twinned data - maybe you ought to use SHELXL to refine it?
Eleanor
john kryst wrote:
Hi all !!
I am working on some deletion mutants. Mutants crystallized in
the same space group with similar cell dimensions. My questions are the
following??
1. Is the free R flag has to be maintained across all the mutants with
respect to the native.
2. Does B-factor has to be flattened (say 30) before starting the
refinement of the each mutant.
3. One of my mutant is twinned. should i maintain the same free R flag
without worrying about the twinning ???
Thanks for your inputs.
with warm regards
John
