Dont forget pointless gives a point group so you could have spacegroup
P4123 too - check that in MR
Could might well have a trimer sitting on the cubic 3-fold, but then the
oligimer will be generated by the crystal symmetry, and you need only
search with a monomer.
Eleanor
Kristof Van Hecke wrote:
Dear all,
When running Pointless 1.2.0 (see summary below), I get the cubic
space group P 4 3 2
(mosflm gave P 2 3 or P 4 3 2)
<!--SUMMARY_BEGIN-->
Best Solution space group P 4 3 2
Reindex operator: [h,k,l]
Laue group probability: 1.000
Systematic absence probability: 0.975
Total probability: 0.975
Space group confidence: 0.964
Laue group confidence 1.000
<!--SUMMARY_END-->
However, although I have multiple models (ensemble) with approximately
30% sequence identity (44% homology), I'm not able to get any
reasonable solution with Phaser.
As we know the protein can form dimers and even trimers, is it
possible that Poinless is giving a higher space group because of
pseudo-symmetry in this case..?
Does anyone has got experience with Pointless and this kind of space
group please..?
Many thanks
Kristof
--------------------------------------
Kristof Van Hecke, PhD
Biomoleculaire Architectuur
Celestijnenlaan 200 F
B-3001 Heverlee (Leuven)
Tel: +32(0)16327477
--------------------------------------
Disclaimer: http://www.kuleuven.be/cwis/email_disclaimer.htm
