Hi Deliang,
We have crystallized the complex cytochrome b6f with an extension of
His6 (no linker) at the c-terminal of cytochrome f (Stroebel, 2003). The
His extension takes part to the crystal contacts. The electron density
is visible but not very well defined. We have not tried to remove the
His6. Many other structure of membrane protein have used His-tag, but
still not enough to make sound statistics.
Daniel
deliang a écrit :
Hi there,
I purified a membrane protein with traditional His-tag on the C
terminal. Before crystallization, I wonder how this tag may affect the
result. Does anyone have experience that the removel of this tag may
improve the result or not? or can provide some references which may give
some statistic, like how many membrane proteins have been crystallized
with or without His-tag?
Thanks so much.
Deliang
