Two structures of NADH complexes. One is a flavoenzyme. I'm sure there are many more.
Pejchal R, Sargeant R, Ludwig ML. Structures of NADH and CH3-H4folate complexes of Escherichia coli methylenetetrahydrofolate reductase reveal a spartan strategy for a ping-pong reaction. Biochemistry. 2005 Aug 30;44(34):11447-57. PMID: 16114881 [PubMed - indexed for MEDLINE Inagaki E, Ohshima N, Takahashi H, Kuroishi C, Yokoyama S, Tahirov TH. Crystal structure of Thermus thermophilus Delta1-pyrroline-5-carboxylate dehydrogenase. J Mol Biol. 2006 Sep 22;362(3):490-501. Epub 2006 Jul 29. PMID: 16934832 [PubMed - indexed for MEDLINE] > From: Buz Barstow <[EMAIL PROTECTED]> > Reply-To: Buz Barstow <[EMAIL PROTECTED]> > Date: Sat, 29 Dec 2007 10:55:32 -0500 > To: <[email protected]> > Subject: [ccp4bb] Crystallographic Experiment with NADH and a Flavoprotein > > Dear All, > > I'm planning an experiment to study the oxidation of NADH by a > flavoprotein at cryogenic temperatures to facilitate collection of X- > ray diffraction data. > > In planning this experiment, I have seen a few obstacles that I am > looking for help in overcoming. > > 1. There are no structures in the PDB that are complexed with NADH or > NAD2H. > Has anyone ever attempted to solve a structure complexed with NADH or > NAD2H, especially at cryogenic temperatures, and if so, what are the > difficulties? Does NAD+ de-bind from the protein too fast to permit > data collection? > > > 2. NADH oxidation typically takes less than a second by a flavoprotein > at room temperature. > Is there an NADH or NAD2H analog that has a much longer half time for > oxidation by a flavoprotein, for example tens of minutes, rather than > tenths of a second, and can this analog still be oxidized at cryogenic > temperatures, with a reasonable half time, of several hours or so? > > > Thanks! and all the best, > > --Buz
